Endocrine Cell Culture [Paperback]

This book offers clear, affordable lab protocols and descriptive accounts of all major endocrine cell cultures.Aimed at postgraduates, researchers and lab professionals, this guide gives in-depth procedures for successfully growing endocrine cells in the lab. This class of cells is used in a range of lab studies since they form an integral part of the hormone system. Each type of cell is given a descriptive account, followed by careful and detailed protocols for successful cultures. Written by experts with first hand experience to avoid common pitfalls, this is a fast route to culturing cells in this important area.Aimed at postgraduates, researchers and lab professionals, this guide gives in-depth procedures for successfully growing endocrine cells in the lab. This class of cells is used in a range of lab studies since they form an integral part of the hormone system. Each type of cell is given a descriptive account, followed by careful and detailed protocols for successful cultures. Written by experts with first hand experience to avoid common pitfalls, this is a fast route to culturing cells in this important area.This handy reference describes the fundamental principles and procedures underlying the successful isolation of viable, functionally intact cells from mammalian endocrine tissues, and their maintenance as primary cultures. The cell types selected for coverage illustrate the diversity of endocrine tissues from which cells have been isolated, and the range of procedures that have been devised to ensure the optimal survival and behavior of each cell type under study. The book places particular emphasis on the provision of detailed protocols describing, step by step, the manipulations necessary to establish differentiated and responsive cultures. The chapters have been prepared by authors who have direct practical experience with the cell types covered, therefore providing the reader with first-hand accounts on the background to each procedure, the avoidancl“.