Epiblast Stem Cells Methods and Protocols [Hardcover]

In Epiblast Stem Cells: Methods and Protocols, expect researchers in the field provide a detailed collection of techniques and protocols useful to the study of the biology of the pluripotent epiblast.? These include methods and techniques used to study epiblast development in different amniotes.?This collection brings together contributions from the fields of embryology, stem cell biology and developmental biology together, providing a single volume with detailed procedures for the isolation and culture of epiblasts at different stages of development, and techniques for the study of differentiation into specific lineages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, a complete list of the necessary materials and reagents, detailed laboratory protocols, and extensive notes providing suggestions on troubleshooting and how to overcome common difficulties.

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Comprehensive and cutting-edge, Epiblast Stem Cells: Methods and Protocols serves as a resource to individuals interested in studying the biology of pluripotent cells.

This book offers a detailed collection of techniques and protocols useful to the study of the biology of the pluripotent epiblast.? It provides step-by-step detail essential for reproducible results and contains expert implementation advice.

1. Generation and Characterization of Epiblast Stem Cells from Blastocyst-stage Mouse Embryos

Daniel C. Factor, Fadi J. Najm, and Paul J. Tesar

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2. Reversion of Mouse Post-Implantation Epiblast Stem Cells to a Na?ve Pluripotent State by Modulation of Signalling Pathways

Astrid Gillich, Siqin Bao, and M. Azim Surani

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3. Converting Mouse Epiblast Stem Celllãf