Epidermal Cells Methods and Protocols [Hardcover]

Reflecting over three decades of advances, Epidermal Cells: Methods and Protocols, Third Edition underscores these advances in our understanding of epidermal biology with updated and entirely new protocols that compliment and extend the earlier edition. The inclusion of protocols useful for both in vitro and in vivo studies reflects many useful developments in the field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Dependable and easy to follow, Epidermal Cells: Methods and Protocols, Third Edition serves researchers working to accelerate the work in this vital field of study.

1. Differentiation of Human Induced Pluripotent Stem Cells into a Keratinocyte Lineage

??????????? Igor Kogut, Dennis R. Roop, and Ganna Bilousova

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2. Differentiation of Epidermal Keratinocytes from Human Embryonic Stem Cells

??????????? Fahad K. Kidwai, Tong Cao, and Kai Lu

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3. Protocol for Serial Cultivation of Epithelial Cells Without Enzymes or Chemical Compounds

??????????? Dongxia Ye and Antonio Peramo

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4. Growth and Differentiation of HaCaT Keratinocytes

??????????? Van G. Wilson

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5. Transgene Delivery to Cultured Keratinocytes Via Replication-Deficient Adenovirus Vectors

??????????? Vincent P. Ramirez and Brian J. Aneskievich

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6. Analyzing the Global Chromatin Structure of Keratinocytes by MNase-Seq

??????????? Jason M. Rizzo and Satrajit Sinha