Epidermal Growth Factor Methods and Protocols [Hardcover]

A comprehensive collection of optimized methods for dissecting the mechanisms that control epidermal growth factors (EGF) and their regulators in both normal and pathological states. These readily reproducible techniques range from the study of purified EGF receptor to complex signaling and processing networks in intact cells, including a chapter on the clinical and pharmacological considerations of their use in cancer therapy. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.A comprehensive collection of optimized methods for dissecting the mechanisms that control epidermal growth factors (EGF) and their regulators in both normal and pathological states. These readily reproducible techniques range from the study of purified EGF receptor to complex signaling and processing networks in intact cells, including a chapter on the clinical and pharmacological considerations of their use in cancer therapy. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.A Historical Perspective of the EGF Receptor and Related SystemsFrancis Edwin, Gregory J. Wiepz, Rakesh Singh, Chimera Rene Peet, Deepti Chaturvedi, Paul J. Bertics, and Tarun B. PatelPurification and Assay of Kinase-Active EGF Receptor From Mammalian Cells by Immunoaffinity ChromatographyGregory J. Wiepz, Arturo G. Guadaramma, David L. Fulgham, and Paul J. BerticsSoluble Epidermal Growth Factor Receptor Acridinium-Linked Immunosorbent AssayJacqueline M. Lafky, Andre T. Baron, and Nita J. MaihleAnalysis of EGF Receptor Interactions lc¶