Exocytosis and Endocytosis [Paperback]

Focused on one of the most important and fascinating fields of modern biology, Exocytosis and Endocytosis, Second Edition presents a comprehensive collection of protocols for cutting edge, recently developed methods of studying exocytosis and endocytosis in vitro and in vivo. Divided into four easy-to-follow sections, chapters focus on diverse research topics including cell-free and biochemical assays of exocytosis and endocytosis, probing protein, membrane and organelle trafficking in cultured cells and powerful in vivo imaging approaches to investigate different physiological processes involving exocytosis and endocytosis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Highly practical and clearly written, Exocytosis and Endocytosis, Second Edition will serve both seasoned researchers as well as newcomers to the field and will provide a unique resource and expert guidance to modern laboratory techniques developed for examining protein and membrane trafficking in simple cellular systems and model organisms.

Part I. An Overview of Methods for Analyzing Vesicular Trafficking

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1. Pharmacological Inhibitors of Exocytosis and Endocytosis: ?Novel Bullets for Old Targets

Andrei I. Ivanov

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2. Systematic Analysis of Endocytosis by Cellular Perturbations

Lena K?hling and Mario Schelhaas

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Part II. Cell-Free and Biochemical Assays of Exocytosis and Endocytosis

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3. Real Time Detection of SNARE Complexes with FREl3Ë