Exosomes and Microvesicles Methods and Protocols [Paperback]

This volume covers methods for the analysis of extracellular vesicles (EV) that can be applied to isolated EVs from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle tracking analysis. The chapters in this book discuss EV cargoes containing proteins and genomic materials using a number of different approaches, and isolating EVs from platelets and neuronal cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and comprehensive, Exosomes and Microvesicles: Methods and Protocols is a valuable resource containing methodologies for anyone interested in researching EVs.

Preface&
Table of Contents&
Contributing Authors&

1.  Methods to Analyze EVs
Bernd Giebel and Clemens Helmbrecht

2. Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles
Sybren L. N. Maas, Marike L.D. Broekman, and Jeroen de Vrij

3. Immuno-Characterization of Exosomes using Nanoparticle Tracking Analysis
Kym McNicholas and Michael Z. Michael

4. Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy
Romain Linares, Sisareuth Tan, C?line Gounou, and Alain R. Brisson

5. Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR
Shayne A. Bellingham, Mitch Shambrook, and Andrew F. Hill

6. Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Tolc7