Extracellular RNA Methods and Protocols [Hardcover]

This volume provides a collection of methods and protocols detailing extracellular RNA and hopes to be a useful resource for those interested in this exciting and emerging field. Chapters are divided into four sections covering an overview of the field, approaches for the isolation of exRNA, protocols for detection and quantitation of exRNA, and approaches that can be useful for studies to evaluate potential therapeutic applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Extracellular RNA: Methods and Protocols aims to ensure successful results in the further study of this vital field.?

1. Extracellular RNAs  A New Awareness of Old Perspectives

Noah Sadik, Lilian Cruz, Alessandra Gurtner, Rodosthenis S. Rodosthenous , Sophie A. Dusoswa, Olivia Ziegler, Thomas Sebastiaan Van Solinge, Zhiyun Wei, Ane Miren Salvador Garicano, Bence Gyorgy, Marike Broekman, and Leonora Balaj

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2. Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles

Julia Small, Roger Alexander, and Leonora Balaj

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3. Extracellular RNA Isolation from Cell Culture Supernatant

Sawen Bakr., Bridget Simonson, Kirsty M. Danielson, and Saumya Das

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4. Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture

Irene K. Yan, Neha Shukla, David A. Borrelli, and Tushar Patel

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5. Isolation of Extracellular Rlӕ