Extracellular Vesicles Methods and Protocols [Hardcover]

This volume examines established methods and protocols to isolate and characterize extracellular vesicles (EVs) and their composition, among other techniques including purification, imaging, biofluid-specific and cell-specific isolation and downstream genomic and proteomic profiling. The international group of expert scientists who have contributed to this collection provide a variety of different techniques related to the growing assortment of EV applications, given that at times using only one technique or two is insufficient to address the question at hand. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?1. Extracellular Vesicles: A Brief Overview and Its Role in Precision Medicine

??????????? Mingyu Shang, John Ji, Chao Song, Bao Jun Gao, Jason Gang Jin, Winston Patrick Kuo, and Hongjun Kang

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2. Red Blood Cells: A Source of Extracellular Vesicles

??????????? Winston Patrick Kuo, John Tigges, Vasilis Toxavidis, and Ionita Ghiran

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Part I: Isolation of Extracellular Vesicles

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3. Isolation of Extracelullar Vesicles by Ultracentrifugation

??????????? Fatemeh Momen-Heravi

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4. Sequential Filtration: A Gentle Method for the IsolatlÓË