This study presents a floristic survey of orchid species occurring in the Serra do Japi in S?o Paulo, southeastern Brazil. The region studied is strategically placed in the transitional zone between interior semi-deciduous mesophytic forests and the Atlantic forest, presenting species from both formations and is characterized by semi-deciduous mesophytic forests, altitudinal forests and rocky outcrops. In the Serra do Japi the orchid family is represented by 125 species distributed among 61 genera. The most representative genus is Epidendrum, followed by Oncidium and Habenaria. Most of the species occur as epiphytes, while 40 species are terrestrial, 31 species are rupicolous, two are hemi-epiphytes and only one is myco-heterotrophic. Although the orchid diversity is high, the region has been affected by anthropogenic disturbances, making the preservation and integrated study of the diversity present in the Serra do Japi an urgent necessity, with social, economic and preservationist aspects.This study presents the floristic survey of orchid species occurring in the Serra do Japi, S?o Paulo, Southeastern Brazil. This region is strategically placed in the transition between interior semi-deciduous mesophytic forests and the Atlantic forest, presenting species from both formations. Although the orchid diversity is high, the region is affected by anthropogenic disturbance. The preservation and the integrated study is an urgent necessity, with social, economic and preservationist reflexes.The observed orchid family includes 125 species distributed among 61 genera. Two new species, Habenaria sp. and Acianthera sp. which were not identified before are described. The most representative genus is Epidendrum, followed by Oncidium and Habenaria. Most of the species occurs as epiphytes, while 40 species are terrestrial, 31 species are rupicolous, two are hemi-epiphytes and only one is a saprophyte. The semi-deciduous mesophytic forest has the highest occurrence of species of Orl[