In Flavins and Flavoproteins: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study flavins and flavoproteins. These include review style methods and protocols to exemplify the variety, the power and the success of modern techniques and methods in application to flavoproteins. Part I of this Volume covers general properties, syntheses and applications of free flavins as well as its analogs and flavoproteins. Part II covers characterizations of flavins and flavoproteins using modern experimental techniques as well as theoretical methods. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Thorough and intuitive, Flavins and Flavoproteins: Methods and Protocols aids scientists in continuing to tackle the countless questions that need to be answered to more fully comprehend the vast diversity and specificity of flavin-governed biological processes.
Part I
1. Structure and General Properties of Flavins
Ana Maria Edwards
2. Recent advances in Riboflavin Biosynthesis
Ilka Haase, Tobias Gr?wert, Boris Illarionov, Adelbert Bacher and Markus Fischer
3. Natural Riboflavin Analogs
Danielle Biscaro Pedrolli, Frank Jankowitsch, Julia Schwarz, Simone Langer, Shinobu Nakanishi and Matthias Mack
4. A Roadmap to the Isotopolog Space of Flavocoenzymes
Adelbert Bacher, Boris Illarionov, Wolfgang Eisenreich and Markus Fischer
5. Electron Transferases
Patricia Ferreira, Marta Mart?nez-J?lvez and Milagros Medina
6. Aldonolactone Oxidoreductases
Nil£p