Flow Cytometry and Cell Sorting [Paperback]

The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories.
A Springer Lab Manual Review of the First Edition:
This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended.
CYTOBIOSFlow cytometric analysis of molecular, biochemical, genetic and developmental parameters using cellular fluorescence techniques as well as fluorescence-activated (FACS) or magnetic (MACS) cell sorting technologies provide unique options for molecular and cellular biology. In recent years, these technologies have been considerably advanced.
In this second edition, all chapters have been updated according to the recent improvements and modifications. Further, new protocols have been added, such as on magnetic selection, magnetofluorescent liposomes, the cytometry of secreted products and microbead assays, as well as reporter gene assays for cytometry and cell selection. The recent technical developments allow diagnostic differentiation of cells according to specific gene expression, identification of rare disease-associated cells and isolation of well-defined cells at high purity for cell therapies.Preface - Leonard A. Herzenberg; Part I Flow Cytometry: Operation of a Flow Cytometer - Christoph G?ttlinger, Birgit Mechtold and Andreas Radbruch; Part II Immunofluorescence: 2 Conjugation of Fluorochromes, Haptens, and Phycobiliproteins to Antibodies - Werner M?ller; 3 Immunofluorescence: Basic Considerations - Andreas Radbruch; 4 Multicolor Immunofluorescence Analysis - Chia-Huei Chen, Kerstin Willmann, Dieter Recktenwald; 5 Combined Intracellular and Surface Staining: Immunofluorescence of Cytokines in T Cells - Mario Assenmacher; 6 Cytometry and Sorting of Live Cells According to Their Sel3‚