Flow Cytometry New Developments [Paperback]

Described here are the practical applications of flow cytometry in specific biological systems, ranging from cell biology to chromosome analysis and sorting. Three major areas of interest in cell and molecular biology are addressed: - Cell Activation and Biological Response. - Membrane-Ligand Interactions and Cell Identity. - Nuclear Components: Form and Function. Data management, expert systems and cell sorting techniques concerning all aspects of flow cytometry are also presented.Proceedings of the NATO Advanced Study Institute 4th International Course on Flow Cytometry held at Villejuif, France, from April 6-10, 1992Described here are the practical applications of flow cytometry in specific biological systems, ranging from cell biology to chromosome analysis and sorting. Three major areas of interest in cell and molecular biology are addressed: - Cell Activation and Biological Response. - Membrane-Ligand Interactions and Cell Identity. - Nuclear Components: Form and Function. Data management, expert systems and cell sorting techniques concerning all aspects of flow cytometry are also presented.I Cell Activation and Biological Response Modifiers.- Cytosolic Events Related to Cell Activation: Potential Interaction Between Signal and Energy Transduction Pathways in Smooth Muscle Cells.- Studies of Changes in Cytoplasmic pH and Membrane Potential Using Flow Cytometry.- Measurement of Enzyme Kinetics by Flow Cytometry.- Endocytosis Analysis by Flow Cytometry: Study of the Endocytosis of Fluoresceinylated Neoglycoproteins via Membrane Lectins of Macrophages.- Analysis of Intracellular Antigens by Flow Cytometry: Methods for Cell Permeabilization to Antibodies.- Expression and Role of Surface Markers of Immunocompetent Cells in Non-lympho?d Systems.- Change in Lipid Composition and in Membrane Fluidity of Human Peripheral Blood Lymphocytes undergoing Blastic Transformation.- II: Membrane - Ligand Interactions and Cell Identity.- Cellular Proliferation History by Fluorl3&