Flow Cytometry A Practical Approach [Paperback]

Flow Cytometry 3/eis intended as a handbook for every laboratory that has a bench-top flow cytometer or a fluorescence activated cell sorter. It is an introduction and guide to those new to the field and a first point of reference for experienced practitioners who want to investigate a new technique. The chapter of immunophenotyping - the most important clinical application of flow cytometry - has been strengthened by the addition of a chapter on quality control in the clinical laboratory. The utility of the book in a clinical laboratory has been further enhanced by the addition of a chapter covering ten other clinical applications. Flow cytometry has found increasing application in the field of apoptosis research. A new chapter has been added to cover this important topic. Every flow cytometry laboratory can't afford not to have a copy on the shelf as a first point of reference. The book is not fully comprehensive but it does aim to cover over 90% of the applications of flow cytometry in mammalian biology.

Preface
List of Protocols
Abbreviations
1. Introduction to the principles of flow cytometry,Nigel P. Carter and Michael G. Ormerod
2. Fluorescence and fluorochromes,Michael G. Ormerod
3. Preparing suspensions of single cells,Michael G. Ormerod
4. Flow sorting,Nigel P. Carter and Michael G. Ormerod
5. Immunofluorescence of surface markers,Michael R. Loken, Cherie I. Green, and Denise A. Wells
6. Analysis of DNA - general methods,Michael G. Ormerod
7. Further clinical applications,Terry Hoy, et al.
8. Quality assurance in the clinical laboratory,Jan W. Gratama and David Barnett
9. Measurement of cytoplasmic and nuclear antigens,Jorgen K. Larsen
10. Analysis of DNA-measurement of cell kinetics by the bromodeoxyuridine/anti-bromodeoxyuridine method,George D. Wilson
11. Analysis of cell proliferation using the bromodeoxyl£P