Flow Cytometry Principles and Applications [Paperback]

Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers.

The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.

Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. Throughout Flow Cytometry: Principles and Applications, well-informed expert contributors present theoretical descriptions and practical protocols on this important and complex laboratory technique and its applications. Immunologists and Hematologists in the field of pathology, as well as biological researchers working with both human and animal models will appreciate the simple, clear-cut style in which principles and protocols in this volume are presented, and will refer to this book time and time again for clear and easy-to-follow protocols.

1 Principles of flow cytometryMarion G Macey1.1 History and development of flow cytometry1.2 Principles of flow cytometry1.3 Fluorescence analysis1.4 Light scatter and fluorescence detection1.5 Acquisition1.6 Amplification1.7 Histograms1.8 Coefficients of variation CV's1.9 Spectral overlap and compensation1.10 Safety aspects of lasers1.11 Cell sorting1.12 Commercial flow cytometers1.13 References2 Cell preparationDesmond A. McCarthy2.1. Introduction2.2. Factors affecting the choice of prepation procedure: live versus fixed samples2.3. Factors affectinlÃ^