Reflecting the expanding fields need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject.? The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS).? Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.
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Comprehensive and practical,
Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both novice and established scientists toward furthering their research with these invaluable techniques.
Part I: Steady-State Fluorescence Spectroscopy (SSFS)
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1. How to Collect National Institute of Standards and Technology (NIST) Traceable Fluorescence Excitation and Emission Spectra
??????????? Adam Matthew Gilmore
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2. Steady State Fluorescence Polarization/Anisotropy for the Study of Protein Interactions
??????????? Nicholas G. James and David M. Jameson
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3. Quantitative Fluorescence Spectral Analysis of Protein Denaturation
??????????? Ivo H.M. van Stokkum and Sergey P. Laptenok
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4. High-Pressure Fluorescence Applications
??????????? Mariano Dellarole and Catherine A. Royer
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