Fluorescence Spectroscopy New Methods and Applications [Paperback]

1. New Methods in Fluorescence Spectroscopy.- 1. Fluorescence Spectroscopy: Where We Are and Where Were Going.- 1 Introduction.- 2 The Marriage of Luminescence Spectroscopy With Separation Methods.- 3 Improvement of Selectivity Based Upon Narrow-Band Excitation.- 3.1 Fluorescence Line Narrowing (FLN) Spectroscopy&.- 3.2 Shpolskii Spectroscopy.- 3.3 Matrix Isolation Fluorescence Spectroscopy.- 3.4 Supersonic Jet Spectroscopy.- 4 Fiber Optic Sensors - Non-Destructive Spectroscopy&.- 5 Semiconductor Lasers.- 6 References.- 2. Interactions and Kinetics of Single Molecules as Observed by Fluorescence Correlation Spectroscopy.- 1 Introduction.- 2 The Experimental Set up.- 3 Theoretical Background.- 4 Results.- 4.1 Translational Diffusion.- 4.2 Rotational Diffusion.- 4.3 Molecular Interactions and Kinetics.- 4.4 Single Molecule Events.- 5 Conclusions.- 6 References.- 3. Fast Optical Imaging Techniques.- 1 Introduction.- 2 High Speed Imaging Technologies.- 2.1 Resolution.- 2.2 Sensitivity.- 2.3 Dynamic Range.- 3 Imaging System Components.- 4 Examples of Detector Systems.- 4.1 Standard Silicon Photodiodes.- 4.2 Vidicon TV Cameras.- 4.3 Charge-Coupled Devices (CCD).- 5 Conclusions.- 4. Kinetic Studies on Fluorescence Probes Using Synchrotron Radiation.- 1 Introduction: Adiabatic Photochemical Product Formation and Fluorescence Probes.- 2 Fluorescence Kinetics and Its Application to TICT States.- 2.1 The Principle of TICT States.- 2.2 The Time Structure of Synchrotron Radiation.- 3 How to Construct TICT Fluorescence Probes.- 3.1 Principal Approaches.- 4 TICT Probes in Biology.- 5 Application of TICT and Other Relaxing Probes in Liquid and Rigid Media.- 6 Proton Transfer Fluorescence Probes.- 7 References.- 5. Dynamics and Geometry in Dimeric Flavoproteins from Fluorescence Relaxation Spectroscopy.- 1 Introduction.- 2 Lipoamide Dehydrogenase and Glutathione Reductase as Biophysical Systems.- 3 Fluorescence Methodology.- 3.1 Molecular Relaxation Spectroscopy.- 3.2 Fluorescencelã#