Fluorescent Analogs of Biomolecular Building Blocks Design and Applications [Hardcover]

Fluorescent Analogs of Biomolecular Building Blocks focuses on the design of fluorescent probes for the four major families of macromolecular building blocks. Compiling the expertise of multiple authors, this book moves from introductory chapters to an exploration of the design, synthesis, and implementation of new fluorescent analogues of biomolecular building blocks, including examples of small-molecule fluorophores and sensors that are part of biomolecular assemblies.

List of Contributors xv

Preface xix

1 Fluorescence Spectroscopy 1
Renatus W. Sinkeldam, L. Marcus Wilhelmsson, and Yitzhak Tor

1.1 Fundamentals of Fluorescence Spectroscopy 1

1.2 Common Fluorescence Spectroscopy Techniques 3

1.2.1 Steady-State Fluorescence Spectroscopy 3

1.2.2 Time-Resolved Fluorescence Spectroscopy 5

1.2.3 Fluorescence Anisotropy 6

1.2.4 Resonance Energy Transfer and Quenching 7

1.2.5 Fluorescence Microscopy and Single Molecule Spectroscopy 8

1.2.6 Fluorescence-Based in vivo Imaging 9

1.3 Summary and Perspective 10

References 10

2 Naturally Occurring and Synthetic Fluorescent Biomolecular Building Blocks 15
Renatus W. Sinkeldam and Yitzhak Tor

2.1 Introduction 15

2.2 Naturally Occurring Emissive Biomolecular Building Blocks 16

2.3 Synthetic Fluorescent Analogs of Biomolecular Building Blocks 18

2.3.1 Synthetic Emissive Analogs of Membranes Constituents 19

2.3.2 Synthetic Emissive Analogs of Amino Acids 22

2.3.3 Synthetic Emissive Analogs of Nucleosides 24

2.4 Summary and Perspective 3l“‘