From Genes to Genomes Concepts and Applications of DNA Technology [Hardcover]

• Increased coverage of whole-genome sequencing technologies and enhanced treatment of bioinformatics.
• Clear, two-colour diagrams throughout.
• A dedicated website including all figures.
  

Noted for its outstanding balance between clarity of coverage and level of detail, this book provides an excellent introduction to the fast moving world of molecular genetics.

1 From Genes to Genomes 1

1.1 Introduction 1

1.2 Basic molecular biology 4

1.2.1 The DNA backbone 4

1.2.2 The base pairs 6

1.2.3 RNA structure 10

1.2.4 Nucleic acid synthesis 11

1.2.5 Coiling and supercoilin 11

1.3 What is a gene? 13

1.4 Information flow: gene expression 15

1.4.1 Transcription 16

1.4.2 Translation 19

1.5 Gene structure and organisation 20

1.5.1 Operons 20

1.5.2 Exons and introns 21

1.6 Refinements of the model 22

2 How to Clone a Gene 25

2.1 What is cloning? 25

2.2 Overview of the procedures 26

2.3 Extraction and purification of nucleic acids 29

2.3.1 Breaking up cells and tissues 29

2.3.2 Alkaline denaturation 31

2.3.3 Column purification 31

2.4 Detection and quantitationlã(