Functional Analysis of DNA and Chromatin [Hardcover]

In Functional Analysis of DNA and Chromatin, expert researchers in the field provide an overview of standard and more recent methods for the functional analysis of the genetic material. These include methods on DNA-dye binding modes, chromatin staining, nuclear dispersion assays, DNA labeling in vivo, sister chromatid exchanges, FISH, DNA and chromatin imaging by fluorescence, electron and atomic force microscopy, detection of apoptotic DNA, cytosine methylation and hydroxy-methylation, DNA thermophoresis, improved methods for histone analysis, chromatin immunoprecipitation, and analysis of rDNA genes and chromatin-associated RNA. Written in the highly successful Methods in Molecular Biology ?series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative?and practical,?Functional Analysis of DNA and Chromatin seeks to aid scientists in the further study of cellular and molecular biology of the nucleus.

Covering standard and recently developed methods in functional analysis of genetic material, this new volume in the Methods in Molecular Biology series includes reproducible protocols, expert problem-solving tips, and improved techniques for histone analysis.

1. Predictive Binding Geometry of Ligands to DNA Minor Groove:

Isohelicity and Hydrogen-Bonding Pattern

Juan C. Stockert

2. Using Microchip Gel Electrophoresis to Probe DNA-Drug Binding Interactions

Nan Shi and Victor M. Ugaz

3. Identifying Different Types of Chromatin using Giemsa Staining

Juan C. Stockert, Alfonso Bl?zquez-Castro, and Richard W. Horobin

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