Functional Genomics Methods and Protocols [Hardcover]

Over the last decade Life Science has undergone an accelerated evolution, culminating in the -omics era characterized by the development of a multitude of high throughput methods that are becoming more routinely applied in biochemistry labs. In Functional Genomics: Methods and Protocols, Second Edition expert researchers in the field detail many of the methods which are now commonly used for studies in the life sciences focusing on the dynamic aspects of the transcriptome, proteome and metabolome, respectively.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Functional Genomics: Methods and Protocols, Second Edition seeks to aid scientists in establishing? or extending technologies and techniques in their laboratories.

In the last decade the life sciences have evolved at a startling rate, culminating in todays high-throughput methods in biochemistry laboratories. In this book, expert researchers focus on dynamic aspects of the transcriptome, proteome and metabolome.

Part I: Bioinformatics

1. Prediction of Protein Tertiary Structures using MUFOLD

Jingfen Zhang, Zhiquan He, Qingguo Wang, Bogdan Barz, Ioan Kosztin, Yi Shang, and Dong Xu

2. Prediction of Protein Functions

Roy D. Sleator

3. Genome Wide Screens for Expressed Hypothetical Proteins

Claus Desler, Jon Ambaek Durhuus and Lene Juel Rasmussen

4. Self Custom-Made SFP Arrays for Non-Model Organisms

Ron Ophir and Amir Sherman

Part II: DNA Anlß