Functional Glycomics Methods and Protocols [Hardcover]

The study of functional glycomics requires the continuous development of rapid and sensitive methods for the identification of glycan structures and integration to structure-function relationships. In Functional Glycomics: Methods and Protocols, a panel of world-renowned experts provide new developments and emerging glycomics techniques in the form of detailed protocols exploring the fundamental challenges and most cutting-edge novel applications. Written in the highly successful Methods in Molecular Biology series format, chapters in this volume present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting key tips on troubleshooting and avoiding known pitfalls.

Authoritative and easy to use, Functional Glycomics: Methods and Protocols serves as an ideal reference for scientists working in biochemistry, molecular biology, cell biology, immunology, microbiology, and virology and a guide to appropriate techniques and the design of achievable research plans in this vital field.

This reference provides the latest developments and emerging techniques used to study functional glycomics. Chapters included needed materials, reagants, step-by-step protocols, notes sections, common pitfalls to avoid and tips on troubleshooting.

1. Functional Glycomics and Glycobiology: An OverviewJianjun Li and James C. Richards2. Historical Overview of GlycoanalysisAlicia M. Bielik and Joseph Zaia3. Quantitative GlycomicsRon Orlando4. Human Gangliosides and Bacterial Lipo-oligosaccharides in the Development of Autoimmune NeuropathiesNobuhiro Yuki5. Biotinylated Multivalent Glycoconjugates for Surface CoatingAlexander Chinarev, Oxana Galanina, and Nicolai Bovin6. Profiling LPS Glycoforms of Non-typeable Haemophilus influenzae by Multiple-Stage Tanl£Y