Presentingstate-of-the-art protocols to study gap junctions, this detailed book firstfocuses on the use of methods and tools to investigate the different aspects ofconnexin expression and gap junction regulation. The second part of the volumedescribes several methods to probe gap junction functionality as such. Writtenin the highly successful Methods in Molecular Biology series format, chaptersinclude introductions to their respective topics, lists of the necessarymaterials and reagents, step-by-step and readily reproducible laboratoryprotocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritativeand practical, Gap Junction Protocols is intended for basic and appliedresearchers in the area of biomedical and life sciences, both in academic andindustrial settings.
1. Analysis of Liver Connexin ExpressionUsing Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction
Micha?l Maes, Joost Willebrords,Sara Crespo Yanguas, Bruno Cogliati, and Mathieu Vinken
2. DNA Methylation Analysis of HumanTissue-Specific Connexin Genes
Xiaoming Liu and Canxia Xu
3. Detection of Connexins in Liver Cells UsingSodiumdodecylsulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis
JoostWillebrords, Micha?l Maes, Sara Crespo Yanguas, Bruno Cogliati, and MathieuVinken
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