Gel Electrophoresis of Proteins A Practical Approach [Paperback]

This new edition is almost a completely new text, with eight of the ten chapters written by new authors. It presents the most reliable methods for essential procedures such as one-dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements of the procedures. In addition, it describes major new techniques developed since the previous edition. These include capillary gel electrophoresis, sequence analysis of gel-resolved proteins, fluorophore-labelled saccharide electrophoresis, and analysis of protein-protein interactions by gel electrophoresis. Like the previous editions, this volume is easy-to-follow and thorough, a laboratory manual writtenbyexperienced researchersforresearchers. The emphasis is on describing the best methods, in step-by-step detail, with copious advice to ensure that each method works the first time. This new edition is a 'must' for anyone currently uses gel electrophoresis.

1. One-dimensional PAGE
2. Protein detection
3. Preparative gel electrophoresis
4. Capillary SDS electrophoresis
5. Conventional isoelectric focusing
6. 2-D gel electrophoresis
7. Peptide mapping
8. Sequence analysis of gel-resolved proteins
9. Analysis of glycoproteins
10. Protein: protein interactions
Appendix 1 - List of suppliers
Appendix 2 - Bibliography of polypeptide detection methods

A laboratory manual presenting methods in a step-by-step format, and hints and tips from experienced researchers. Among the varieties of gel electrophoresis described are one-dimensional polyacrylamide; isoelectric, two-dimensional; and preparative. Eight of the ten chapters of the earlier editions, in 1981 and 1990, have been rewritten by new authors. They discuss methods that have been developed and tested since then, including capillary gel electrophoresis, the sequence analysis of gelló0