Gene Cloning and Manipulation [Paperback]

An updated edition of this concise guide to recombinant DNA techniques for advanced undergraduates.Fully updated to reflect recent advances, this introduction provides a broad but concise coverage of recombinant DNA techniques. It is written specifically for students and researchers who want to use this technology, with emphasis placed on understanding the underlying concepts, thereby enabling the reader to devise their own strategies.Fully updated to reflect recent advances, this introduction provides a broad but concise coverage of recombinant DNA techniques. It is written specifically for students and researchers who want to use this technology, with emphasis placed on understanding the underlying concepts, thereby enabling the reader to devise their own strategies.Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.Preface; 1. The tools for the job; 2. Polymerase chain reaction; 3. Simple cloning; 4. Other vector systems for E. Coli; 5. Making libraries; 6. Screening libraries; 7. Modification and mutagenesis; 8. Use of cloned DNA; 9. Using other organisms; 10. Examples.