Gene Correction Methods and Protocols [Hardcover]

Gene correction is a technology that gives us the tools for both repairing and mutating DNA, for discovering gene functions and for engineering new genetic variants. Gene Correction: Methods and Protocols provides a user friendly, detailed and up-to-date collection of strategies and methodologies utilized for generating specific sequence changes in the DNA of cells in the laboratory, while also tackling the major problems that the field of gene correction faces. This volume brings together many experts in the field of gene correction to disclose a wide and varied array of specific gene correction protocols for engineering mutations in DNA, for delivering correcting DNA to target cells, and for improving the accuracy and safety of the gene correction process. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Gene Correction: Methods and Protocols seeks to serve scientists of all backgrounds interested in the area of gene targeting/recombination/therapy.

Part I. Approaches for Gene Correction from Bacteria to Human Cells

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1. RecTEPsy Mediated Recombineering in Pseudomonas syringae

??????????? Bryan Swingle

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2. Genome Manipulations with Bacterial Recombineering and Site-Specific Integration in Drosophila

Yi Zhang, William Schreiner, and Yikang S. Rong

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3. Multiple Genetic Manipulations of DT40 Cell Line

Akira Motegi and Minoru Takata

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4. Gene Targeting of Human Pluripotent Stem Cells by Homologous Recombinatilsh