Gene Probes Principles and Protocols [Paperback]

Senior scientists Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using genes probes in a wide variety of applications. The applications covered range from searching for specific genes in the human chromosome to the detection of microorganisms and their toxins in the environment and in food samples, as well as in the diagnosis of human disease. Helpful tutorials explain the principles of gene probe design, labeling, detection, target formation, and hybridization conditions.Target Format and Hybridization ConditionsAlex ReidEnd Labeling Procedures: An OverviewElena HilarioPhotobiotin LabelingElena HilarioNick TranslationAlex ReidRandom Primed LabelingAlex ReidDesign and Evaluation of 16S rRNA-Targeted Oligonucleotide Probes for Fluorescence In Situ HybridizationPhilip Hugenholtz, Gene W. Tyson, and Linda L. BlackallEvaluation of Alterations in the Tumor Supressor Genes INK4A and INK4B in Human Bladder TumorsIrene Orlow and Carlos Cordon-CardoThe b-Tubulin Gene Region as a Molecular Marker to Distinguish Leishmania ParasitesAlexis Mendoza-Le?n, Luis Luis, and Clara Mart?nezDetection of Malignant Plasma Cells in the Bone Marrow and Peripheral Blood of Patients with Multiple MyelomaRoss D. Brown and P. Joy HoDetection of Chlamydia trachomatis by DNA AmplificationPeter Timms and Sarah MathewsIsolation and Identification of Campylobacter Species by Use of Selective Enrichment, Nucleic Acid Amplification Methods, and Gene ProbesAlex van Belkum, Nicole van den Braak, Leen-Jan van Doorn, and Hubert EndtzDiagnosis of Human Papillomavirus Using In Situ Hybridization and In Situ Polymerase Chain ReactionGerard J. NuovoQuantitation of In Situ Hybridization Using Image Analysis of Radioactively Labeled RNA ProbesTeresa Bisucci, Tim D. Hewitson, and Ian A. DarbyStable Isotope Probing for Detection of Methanotrophs After Enrichment with 13CH4Stefan Radajewski and J. Colin MurrellDetection of Enterocl³‹