In this volume of Methods in Molecular Biology , expert investigators offer comprehensive, complementary, and cutting-edge technologies for studies of gene regulation. The chapters of Gene Regulation: Methods and Protocols are organized to provide an integrated and a coherent view of control systems and their associated components. The protocols are broad in their scope. They include molecular, biochemical, spectroscopic techniques as well as high throughput strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Comprehensive and broad in their scope, the protocols are useful to researchers in many disciplines including molecular biology, genomics, biochemistry, biomedicine, nutrition, and agricultural sciences.
This volume offers cutting-edge technologies for studies of gene regulation. The chapters include introductions to their respective topics, lists of the necessary materials and reagents, and step-by-step, readily reproducible laboratory protocols.
1. Gene Regulation
Minou Bina
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2. Isolation of Nuclei for use in Genome-wide DNase Hypersensitivity Assays to Probe Chromatin Structure
Guoyu Ling and David J. Waxman
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3. DNase I Digestion of Isolated Nulcei for Genome-Wide Mapping of DNase Hypersensitivity Sites in Chromatin
Guoyu Ling and David J. Waxman
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4. Isolation and Analysis of DNA Derived from Nucleosome-Free Regions
Matthew Murtha, Yatong Wang, Claudio Basilico, and Lisa Dailey
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5. Acquisition of High l£)
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