Gene Therapy of Solid Cancers Methods and Protocols [Hardcover]

This volume provides insight into recent developments on experimental and clinical strategies for cancer gene therapy. Gene Therapy of Solid Cancers: Methods and Protocols guides readers through protocols on gene therapeutic strategies in combination with helpful technical notes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Concise and easy-to-use, Gene Therapy of Solid Cancers: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Part I: Immunogene Therapy

1. Aptamer Targeting the ERBB2 Receptor Tyrosine Kinase for Applications in Tumor Therapy

Georg Mahlknecht, Michael Sela, and Yosef Yarden

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2. Gene Gun Her2/neu DNA Vaccination: Evaluation Of Vaccine Efficacy in a Syngeneic Her2/neu Mouse Tumor Model

Tam Nguyen-Hoai, Antonio Pezzutto, and J?rg Westermann

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3. MIDGE Technology for the Production of a Fourfold Gene-Modified, Allogenic Cell-Based Vaccine For Cancer Therapy

Manuel Schmidt, Barbara Volz, Patrick Gro?mann, Kerstin Heinrich, and Burghardt Wittig

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Part II: Suicide Gene Therapy

4. Evaluation of Bystander Cell Killing Effects in Suicide Gene Therapy of Cancer: Engineered Thymidylate Kinase (TMPK)/AZT Enzyme-Prodrug Axis

Takeya Sato, Anton Neschadim, Ryo Nakagawa, Teruyuki Yanagisawa, and Jeffrey A. Medin

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5. Oncoleaking: Use of the Pore-forming Clostridium perfringens Enterotoxin (CPE) for Sló`