General Techniques of Cell Culture [Paperback]

Concise introduction to a major technique of cell biology laboratories for those new to the field.Covering the basic essentials for growing cells outside the body, this handbook is aimed at those carrying out tissue cell culture for the first time, or researchers looking for a concise reference to this much used technique. Advice is given on the right equipment, solutions and ingredients necessary to prepare a medium in which cells will grow. Step by step guides are included which explain how to count, grow and store cells, how to tell if cells are growing healthily and what to do if things go wrong. Written in accessible style for those new to the field.Covering the basic essentials for growing cells outside the body, this handbook is aimed at those carrying out tissue cell culture for the first time, or researchers looking for a concise reference to this much used technique. Advice is given on the right equipment, solutions and ingredients necessary to prepare a medium in which cells will grow. Step by step guides are included which explain how to count, grow and store cells, how to tell if cells are growing healthily and what to do if things go wrong. Written in accessible style for those new to the field.Cell culture techniques are invaluable to the modern researcher but difficult to carry out successfully. As part of the series of Handbooks in Practical Animal Cell Biology, this volume offers a concise practical guide to the basic essentials of the technique. Researchers new to cell culture will find a clear explanation of the essential equipment of a tissue culture facility, including tissue culture media and sera. It describes methods for growing suspension and adhesion cultures, including how to store cells and prepare primary cultures from cells. For those already culturing cells, the handbook will act as a handy reference to the basic techniques. The essence of the book is to deal with the generalities of cell culture and to give a grasp of the basic conlÃ*