Genetic Transformation Systems in Fungi, Volume 1 [Paperback]

Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples used in laboratories worldwide. 

Not only will this text provide a detailed background on applications in industrial and pharmaceutical relevant microbes, but also the importance of fungal pathogens in agricultural production (Phytophthora and Botrytis) and mammalian infection (Penicillium marneffei and Candida). Genetic Transformation Systems in Fungi, Volume 1 provides in-depth coverage of how the transformation of DNA is used to understand the genetic basis behind these fungal traits.

Preface

Contributors

I.?????????????????? Introduction

1.????? Fungal Transformation: From Protoplasts to Targeted Recombination Systems

Juan F. Mart?n

II.??????????????? Transformation Methods: Protoplast Transformation

2.????? Protoplast Transformation for Genome Manipulation in Fungi

Aroa Rodriguez-Iglesias and Monika Schmoll

3.????? Trichoderma Transformation Methods

M?nica G. Malmierca, Rosa E. Cardoza and Santiago Guti?rrez

4.????? Transformation of Mucor circinelloides f. lusitanicus Protoplasts

Victoriano Garre, Jos? Luis Barredo and Enrique A. Iturriaga

5.????? Transformation of Saccharomyces cerevisiae: Spheroplast Method

Shigeyuki Kawai and Kousaku Murata

III.???????????? Transformation Methods: Electroplc)