Genetic Transformation Systems in Fungi, Volume 2 [Paperback]

Fungi are an economic very important class of microbes. Not only do they host a range of versatile enzymes used in industrial applications (biofuels, laundry, food processing), as well do they produce several very important pharmaceutical drugs (statins and penicillins). Moreover, fungal pathogens can cause great damage in agricultural production (Phytophthora and Botrytis) and during mammalian infections (Penicillium marneffei and Candida). Transformation of DNA is used to understand the genetic basis behind these traits. Several different techniques have been developed over the years and readily shown to be decisive methods to improve fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples to be used in laboratories worldwide.

I.?????????????????? Endogenous DNA: Cell Fusion

1.????? Anastomosis and Heterokaryon Formation

Martin Weichert and Andr? Flei?ner

2.????? Induction of the Sexual Cycle in Filamentous Ascomycetes

Jos Houbraken and Paul S. Dyer

3.????? What Have We Learned by Doing Transformations in Neurospora tetrasperma?

Durgadas P. Kasbekar

II.??????????????? Endogenous DNA: Repetitive Elements

4.????? Repeat-induced Point Mutation: A Fungal-specific, Endogenous Mutagenesis Process

James K. Hane, Angela H. Williams, Adam P. Taranto, Peter S. Solomon and Richard P. Oliver

5.????? Calculating RIP Mutation in Fungal Genomes Using RIPCAL