This second edition provides updated and new chapters on selected genetic, molecular, biochemical, and cell biological techniques. Chapters guide readers through methods and principles on primordial germ cells and germline stem cells, however many of these principles can be applied to different types of adult stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Germline Stem Cells: Second Edition aims to present the new findings and techniques that have developed since the first edition.
Sarah L. Crittenden, Hannah S. Seidel, and Judith Kimble
2. Methods for Studying the Germline of the Human Parasite Schistosoma Mansoni
Julie N.R. Collins and James J. Collins III
3. Evaluation of the Asymmetric Division of Drosophila Male Germline Stem Cells
Mayu Inaba and Yukiko M. Yamashita
4. Live Imaging of the Drosophila Testis Stem Cell Niche
Leah J. Greenspan and Erika L. Matunis
5. RNA Isolation from Early
Drosophila Larval Ovaries
Dana Gancz and Lilach Gilboa
6. Live-Cell Imaging of the Adult Drosophila Ovary using Confocal Microscol¹