Focusing on new methods and techniques developed to address longstanding questions about the Golgi complex, this volume explores a diverse set of chapters, ranging from live and fixed cell imaging techniques to
in vitro biochemical reconstitution systems. Each chapter provides a detailed set of specific instructions, which should enable anyone to successfully complete the assays. Written for the highly successful
Methods in Molecular Biology series program, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips from the experts on troubleshooting and avoiding known pitfalls.
Practical and detailed, The Golgi Complex: Methods and Protocols will aid both new and established researchers in the field by providing strong hands-on instructions that can be directly applied to their research programs.
1. 4D Confocal Imaging of Yeast Organelles
Kasey J. Day, Effrosyni Papanikou, and Benjamin S. Glick
2. Imaging the Polarized Sorting of Proteins from the Golgi Complex in Live Neurons
Ginny G. Far?as, Dylan J. Britt, and Juan S. Bonifacino
3. Imaging Golgi Outposts in Fixed and Living Neurons
Mariano Bisbal, Gonzalo Quassollo, and Alfredo Caceres
4. Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor
Jon E. Paczkowski and J. Christopher Fromme
5. l#œ
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