This third edition volume expands on the previous editions with new protocols and up-to-date techniques to help researchers excel in this field. The chapters in this book cover topics such as ELISpots sibling (FluoroSpot); ELISpot for influenza, Dengue virus, and tuberculosis; and vaccine research, essentials control, and assay evaluation in ELISpot. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Handbook of ELISPOT: Methods and Protocols, Third Edition is a valuable resource for both novice researchers and experienced ELISpot users.
Preface&
Table of Contents&
Contributing Authors&
1. Challenges in Developing Protein Secretion Assays at a Single-Cell Level
Yoshitaka Shirasaki and Osamu Ohara
2. To Count of Not to Count: ELISPOT Evaluation Revisited
Sylvia Janetzki
3. Essential Controls for ELISpot Assay
Jodi Hagen and Alex Kalyuzhny
4. Automatic Search of Spots and Color Classification in ELISPOT Assay
Sergey S. Zadorozhny and Nikolai N. Martynov
5. Four Color ImmunoSpot ? Assays for Identification of Effector T Cell Lineages
Jodi Hanson, Diana R. Roen, and Paul V. Lehmann
6. Detection of Cross-Reactive B Cells Using the Fluorolă
![Handbook of ELISPOT Methods and Protocols [Hardcover]](/itemImage/2/2/4/7/2_1361854.jpg)