Handbook of Proteomic Methods [Paperback]

A compendium of thirty-four powerful techniques for identifying and analyzing the diversity of proteins expressed in cells. Thee readily reproducible proteomic methods range from general to specific techniques, and include methods for data analysis, posttranslational modification, and its variants and isoforms. Additional methods demonstrate the application of proteomics to the discovery of serological tumor markers, to identifying the determinants of sensitivity to antitumor drugs, and to specialized fields, such as endocrinology, plant biology, nephrology, and urology.A compendium of thirty-four powerful techniques for identifying and analyzing the diversity of proteins expressed in cells. Thee readily reproducible proteomic methods range from general to specific techniques, and include methods for data analysis, posttranslational modification, and its variants and isoforms. Additional methods demonstrate the application of proteomics to the discovery of serological tumor markers, to identifying the determinants of sensitivity to antitumor drugs, and to specialized fields, such as endocrinology, plant biology, nephrology, and urology.Part I. General TechniquesProteomics and the Molecular BiologistPaul R. Graves and Timothy A. J. HaysteadProtein Identification from 2-D Gels Using In Vitro Transcription Translation ProductsNathalie Norais, Renzo Nogarotto, Emilia Tiziana Iacobini, Ignazio Garaguso, Renata Grifantini, Giuliano Galli, and Guido GrandiSelective Chemical Cleavage Methods in Proteomics, Including C-Terminal Successive DegradationAkira TsugitaMeans of Hydrolyzing Proteins Isolated upon ProteinChip? Array Surfaces: Chemical and Enzymatic ApproachesShanhua Lin, Ning Tang, and Scot R. WeinbergerA Combined Radiolabeling and Silver Staining Technique for Improved Visualization and Localization of Proteins on Two-Dimensional GelsJules A. Westbrook and Michael J. DunnQualitative and Quantitative Proteomic Analyses via Multidimensional Protein Identification Techl³8