Haplotyping Methods and Protocols [Paperback]

This volume details protocols for genetic, molecular, cytological, and bioinformatic methods for determining haplotypes.  Haplotyping: Methods and Protocols guides readers through methods that directly type haploid cells, difficult-to-resolve gene families, high-resolution, short range haplotyping for targeted loci, and long-range haplotyping for whole chromosomes or genomes.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical Haplotyping: Methods and Protocols, aims to provide researchers with an overview of experimental methods for haplotyping.

Part I: Haplotyping with Long Range PCR

 

1. Haplotyping of Heterozygous SNPs in Genomic DNA using Long Range PCR

Barbara Arbeithuber, Angelika Heissl, and Irene Tiemann-Boege

 

2. Quantification and Sequencing of Crossover Recombinant Molecules from Arabidopsis Pollen DNA

Kyuha Choi, Nataliya E. Yelina, He?di Serra, and Ian R. Henderson

 

Part II: Selective Sequencing of Gene Families (including the MHC)

3. Pacbio for Haplotyping in Gene Families

Wei Zhang and Joachim Messing

 

4. High Molecular Weight DNA Enrichment with Peptide Nucleic Acid Probes

 

5. High-Throughput Sequencing of the Major Histocompatibility Complex following Targeted Sequence Capture