Hedgehog Signaling Protocols [Paperback]

Intended for Molecular Biologists, Biochemists, and Geneticists interested in manipulating and analyzing the Hedgehog Signaling Pathway, this volume offers various detailed chapters on experimental description, including anecdotal pointers and notes.

In both vertebrates and invertebrates, Hedgehog signaling regulates the development of the tissues and structures of the body. In addition, many cancers and diseases arise as a result of misregulation of the pathway, which makes it an incredibly important system to understand. This volume examines various methods used in the analysis of the Hedgehog signaling pathway, bringing together different lines of analyses into one accessible and comprehensive text. This volume provides a single source for investigators, presenting several different experimental approaches, which are all varied and broad in nature, as well as procedures for a variety of different model systems. Researchers will find this volume to be an invaluble tool as they work towards deeper understanding of the underlying molecular events that the Hedgehog signaling pathway regulates. 

TABLE OF CONTENTSChapter 1. Purifying the Hedgehog protein and its variants by Darren P.Baker, Frederick R. Taylor and R. Blake PepinskyChapter 2. Application of Sonic hedgehog to the developing chick limbby Eva Tiecke and Cheryll TickleChapter 3. Manipulation of Hedgehog signaling in Xenopus by means ofembryo microinjection and application of chemical inhibitors by ThomasHollemann, Emmanuel Tadjuidje, Katja Koebernick & Tomas PielerChapter 4. Isolation of rat telencephalic neural explants to assay ShhGABAergic interneuron differentiation-inducing activity by Rina Madyand Jhumku D. KohtzChapter 5. Genetic Analysis of the Vertebrate Hedgehog SignalingPathway Using Muscle Cell Fate Specification in the Zebrafish Embryo bySudipto RoyChapter 6.lƒ#