Contents.- II. The use of mutants in the study of metabolism.- I. Introduction.- II. Methods.- 1. Isolation of mutant organisms.- 2. Principles underlying the use of mutants.- A. Phenomena after a block.- B. Phenomena before a block.- C. Phenomena at a block.- 3. Comments on mutant methods.- III. Illustrations of mutant techniques.- 1. Amino acid biosynthesis.- A. Classification of amino acids.- B. Glutamic family.- a) Glutamic acid to proline.- b) Glutamic acid to ornithine.- c) Distinction between exogenous and endogenous ornithine.- d) Ornithine to arginine.- e) Ornithine path in Escherichia coli.- C. Aspartic family.- a) Aspartate to homoserine.- b) Homoserine to methionine.- c) Homoserine to threonine and isoleucine.- D. Lysine.- a) Lysine formation in fungi.- b) Diaminopimelic acid and lysine synthesis in E. coli.- E. Pyruvic family.- a) Valine.- b) Leucine.- F. Serine family.- a) Serine and glycine.- b) Cysteine.- G. Histidine.- a) Phosphate ester intermediates.- b) Histidinol to histidine.- H. Shikimic family.- a) Shikimic acid and the aromatic amino acids.- b) 2-Keto-3-deoxy-7-phospho-d-glucoheptonic acid and the formation of shikimic acid.- c) Phenylalanine.- d) Tyrosine.- e) Tryptophan.- 2. Vitamin biosynthesis and miscellaneous applications.- A. Niacin.- a) Tryptophan as niacin precursor.- b) Tryptophan to kynurenine.- c) Kynurenine to 3-hydroxyanthranilic acid.- d) 3-Hydroxy-anthranilic acid to niacin.- B. Pantothenic acid.- a) Pantoic acid.- b) ?-Alanine and pantothenic acid.- C. Other vitamins.- D. Miscellaneous applications.- a) Purines and pyrimidines.- b) Pigments and bioluminescence.- c) Acetate metabolism.- d) Amino-group metabolism.- e) Inorganic metabolism.- IV. Concluding remarks.- Literature.- III. Der Erwerb von Wasser und Mineralstoffen bei Hemiparasiten.- I. Einleitung, anatomische Voraussetzungen.- II. Wasseraufnhme.- III. Mineralsalzern?hrung.- Literatur.- IV. Physiologisch-chemische Fragen der Heterotrophie.- A. Methoden der Kultur mil“ơ