Histological Analysis of Endocrine Disruptive Effects in Small Laboratory Fish [Hardcover]

• Timely title assembling the combined knowledge of some of the leading authorities in the field of small fish reproduction - an important topic for risk assessment and registration of chemical, agricultural, and pharmaceutical compounds
• Provides guidance on the microscopic structure of living tissue and evaluation of the reproductive glands of small laboratory fish
• Includes state-of-the-art science along with sufficient anatomical and physiological background for understanding and interpreting test results
• Helps standardize the interpretation of results from aquatic bioassays and field observations, which will also clarify inconsistencies in the current scientific literature

Note: CD-ROM/DVD and other supplementary materials are not included as part of eBook file.

Preface xi

Acknowledgments xiii

Contributing Authors xv

1 Introduction 1

References 3

2 Fish Species of Interest 7

2.1 Fathead Minnow (Pimephales promelas) 8

2.2 Medaka (Oryzias latipes) 10

2.3 Zebrafish (Danio rerio) 12

2.4 Other Fish Species 13

References 15

3 Sexual Determination, Differentiation, and Gonadal Development 19

3.1 Primordial Germ Cells in the Primordial (Primary) Gonad 22

3.1.1 Differentiation and Number of PGCs 22

3.1.2 Molecular Markers of PGCs 25

3.2 Reproductive Strategies 26

3.3 Differentiation of the Primordial Gonad into Ovary or Testis 27

3.4 Gonadal Duct Formation 32

3.5 Endocrinologyls-