Host-Bacteria Interactions Methods and Protocols [Hardcover]

Host-Bacteria Interactions: Methods and Protocols details cutting edge protocols that cover aspects of the investigation of host bacteria interactions using mammalian and novel non mammalian infection models, cell biology, OMICS and bacterial genetics. Chapters focus on techniques that can be used to investigate different aspects of the physiopathology of bacterial infections, from the whole animal to tissue, cellular and molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Host-Bacteria Interactions: Methods and Protocols provide researchers with a comprehensive account of the practical steps necessary for carrying out each protocol successfully.

Part I: Infection Models to Study Bacterial Virulence

1. Galleria mellonella as an Infection Model for Select Agents

Nicolas Sprynski^, Eric Valade, ?and Fabienne Neulat-Ripoll

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2. Drosophila as a Model for Intestinal Infections

Matthieu Lestradet, Kwang-Zin Lee, and Dominique Ferrandon

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3. Zebrafish Embryos as a Model to Study Bacterial Virulence

Jennifer Mesureur and Annette C. Vergunst

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4. Studying Host-pathogen Interaction Events in Living Mice Visualized in Real Time using Bio photonic Imaging

Gary Splitter, Jerome Harms, Erik Petersen, Diogo Magnani, Marina Durward, Gireesh Rajashekara, and Girish Radhakrishnan