Human Embryonic Stem Cells Handbook [Hardcover]

Considerable advances have taken place since the initial isolation and characterization of human embryonic stem (HES) cells; however, significant challenges remain before their potential for restoration and regeneration processes in patients can be realized. Understanding the diversity amongst HES cell lines and realizing the ability to isolate lines with robust differentiation potential remain difficult.? In the Human Embryonic Stem Cells Handbook, experts in the field provide an assortment of protocols that have been used by various laboratories around the world so as to allow both novices and experienced investigators to compare and contrast different approaches to HES cell isolation and characterization with the hope that, from these protocols, researchers might standardize approaches for HES cell biology.? Written in the Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

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Stem cell research has achieved much, yet still faces many challenges. In this comprehensive handbook, experts provide step-by-step, proven protocols allowing both novices and experienced researchers to make a comparative assessmentand avoid common pitfalls.

1. Establishment of New Lines of Human Embryonic Stem Cells: Evolution of the Methodology

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2. Successful Scale-Up andlÃ-