Human Papillomaviruses Methods and Protocols [Paperback]

Leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of the human papillomavirus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze papillomavirus proteins. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.Leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of the human papillomavirus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze papillomavirus proteins. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.Identification of New Papillomavirus TypesEthel-Michele de Villiers, Corinna Whitley, and Karin GunstIdentification of HPV VariantsJohn Cason, Jon Bible, and Christine MantHistochemical Analysis of Cutaneous HPV-Associated LesionsKiyofumi EgawaHistological Analysis of Cervical Intraepithelial NeoplasiaMichael Babawale, Rashmi Seth, Adam Christian, Wessam Al-Utayem, Ragini Narula, and David JenkinsDetection of Papillomavirus Proteins and DNA in Paraffl#ï