The Human Virome Methods and Protocols [Hardcover]

This book details the most comprehensive, up-to date, and cutting-edge protocols used in wet and dry labs to investigate the viral communities harbored within and on the human body. Chapters guide readers through methods on collection, isolation, identification and computational/statistical analysis, and body niches to cover those methodological issues inherent to the human tissues and organs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, The Human Virome: Methods and Protocols aims to facilitate researchers with their daily work in the field of the research on the human virome.

1. EM Tomography of LINC Complexes

Marie-Christin Spindler, Frederik Helmprobst, Christian Stigloher, and Ricardo Benavente

 

Victor E. Cruz and Thomas U. Schwartz

 

3. Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis

Chloe Potter and Didier Hodzic

 

4. Analysis of Nesprin-2 Interaction with its Binding Partners and Actin

 

5. Interactions of Nesprin-4-containing LINC Complexes in Outer Hair Cells Explored by BioID

Brian Burke

 

6. Using Nesprin Tension Sensors to Measure Force on the LINC Complex

Paul T. Arsenovic and DanilSŠ