Hypoxia Methods and Protocols [Hardcover]

This volume details widely used and newer lab?protocols for studying hypoxic responses in?physiology and diseases. Chapters guide the?reader through the application of hypoxic?conditions, to the techniques of molecular?biology, biochemical, cell biology, genomic,bioinformatic, metabolic, and animal?studies. Written in the highly?successful Methods in Molecular Biology series?format, chapters include introductions to their?respective topics, lists of the necessary?materials and reagents, step-by-step, readily?reproducible laboratory protocols, and tips on?troubleshooting and avoiding known pitfalls.?

Authoritative and cutting-edge, Hypoxia: Methods?and Protocols aims to provide a valuable set of?tools that can be used to study hypoxia and?beyond.

1. Whole Genome Sequencing-based Discovery of Structural Variants in Glioblastoma

Kazimierz O. Wrzeszczynski, Vanessa Felice, Minita Shah, Sadia Rahman, Anne-Katrin Emde, Vaidehi Jobanputra, Mayu Frank, and Robert B. Darnell

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2. Whole Genome DNA Methylation Analysis of Human Glioblastoma using Illumina BeadArrays

Jonathan Serrano and Matija Snuderl

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3. Establishing Primary Human Glioblastoma Adherent Cultures from Operative Specimens

Amit D. Gujar, Diane D. Mao, John B. Finlay, and Albert H. Kim

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Joshua Frenster and Dimitris Placantonakis

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5. Isolation of GBM Stem Cells with Flow Cytometry

Julio Inocencio, Joshua Frenster, and Dimitris Placantonakis

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