Imaging Flow Cytometry Methods and Protocols [Hardcover]

This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells.? Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.

Part I: General

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1. Quantitative Functional Morphology by Imaging Flow Cytometry

??????????? Ivan A. Vorobjev and Natasha S. Barteneva

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2. Principles of Amnis Imaging Flow Cytometry

??????????? David A. Basiji

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3. Ultrafast Microfluidic Cellular Imaging by Optical Time-Stretch

??????????? Andy K.S. Lau, Terence T.W. Wong, Ho Cheung Shum, Kenneth K.Y. Wong, and Kevin K. Tsia

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4. Applications of Imaging Flow Cytometry for Microalgae

??????????? Mark Hildebrand, Aubrey Davis, Raffaela Abbriano, Haley R. Pugsley, Jesse C. Traller, Sarah R. Smith, Roshan P. Shrestha, Orna Cook, Eva Sanchez, Kalpana Manandhar-Shrestha, and BlSÃ