Imaging and Tracking Stem Cells Methods and Protocols [Hardcover]

Imaging and Tracking Stem Cells: Methods and Protocols gathers representative protocols related to the vital techniques of stem cell imaging and lineage tracing, including that of live cells, both in vivo and in vitro. The detailed chapters presented within have been validated for reproducibility and are described in an easy to follow, step-by-step fashion so as to be valuable for not only experts but also novices in the stem cell field.? As with other volumes in the highly successful Methods in Molecular Biology series, chapters conclude with a Notes section, which provides tips on troubleshooting and avoiding known pitfalls.

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Authoritative and practical, Imaging and Tracking Stem Cells: Methods and Protocols provides both a flavor of the field as it currently is and a source to stimulate new approaches and methodologies by those interested in tracking stem cells and their progeny.

This book presents?key methods and protocols aimed at exploring?the imaging and tracking of a wide?variety?of stem cells. It provides step-by-step detail essential for reproducible results as well as offers key implementation advice.

1. Primary Culture and Live-Imaging of Adult Neural Stem Cells and Their Progeny

??????????? Felipe Ortega, Benedikt Berninger, and Marcos R. Costa

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2. Labeling and Tracking of Human Mesenchymal Stem Cells Using Near-Infrared Technology

??????????? Marie-Therese Armentero, Patrizia Bossolasco, and Lidia Cova

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3. High Content Imaging and Analysis of Pluripotent Stem Cell-Derived Cardiomyocytes

??????????? G?bor F?ldes and Maxime Mioulane

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4. A High Content Imaging-Based Approach for Classifying Cellular Phenotypes

??????????? Joseph J. Kim, Selӻ