Immobilized Biocatalysts An Introduction [Paperback]

Biotechnological processes often require the use of immobilized biocatalysts. This translation of the most successful German edition introduces the theoretical background of this rapidly growing field followed by a detailed description of the various techniques of immobilizing, characterizing, and using biocatalysts. The comprehensive discussion of possible applications in industrial processes and in basic research results in a practical guide for everyone involved in these techniques. Furthermore, some selected experiments are added in order to facilitate the understanding of the theoretical reports.Biotechnological processes often require the use of immobilized biocatalysts. This translation of the most successful German edition introduces the theoretical background of this rapidly growing field followed by a detailed description of the various techniques of immobilizing, characterizing, and using biocatalysts. The comprehensive discussion of possible applications in industrial processes and in basic research results in a practical guide for everyone involved in these techniques. Furthermore, some selected experiments are added in order to facilitate the understanding of the theoretical reports.Theoretical Section.- 1 General Principles.- 1.1 Principles of Biocatalysis.- 1.2 Structure of Enzymes.- 1.3 Classification and Nomenclature of Enzymes.- 1.4 Definition and Classification of Immobilized Biocatalysts.- 1.5 Reasons for Immobilization.- 1.6 The History of Immobilization.- 1.7 Economic Importance.- 2 Methods of Immobilization.- 2.1 Adsorption.- 2.2 Ionic Binding.- 2.3 Covalent Binding.- 2.4 Cross-linking.- 2.5 Matrix Entrapment.- 2.6 Membrane Confinement.- 2.7 Combined Methods.- 2.8 Immobilization of Coenzymes.- 3 Characteristics of Immobilized Biocatalysts.- 3.1 Activity as a Function of Temperature.- 3.2 Stability as a Function of Temperature.- 3.3 Temperature Optimum in Long-term Processes.- 3.4 The Influence of pH Value.- 3.5 The Influence of Substrate ConclƒŒ