The Immune Synapse Methods and Protocols [Paperback]

This volume provides all the essential protocols that are currently used to study the immune synapse. Chapters in The Immune Synapse: Methods and Protocols cover methods for the study of the dynamics of immune synapse assembly, traffic at the immune synapse, new high resolution imaging, biophysical and computational methods for the study of the immune synapse, effector immune synapses, B cell, NK and mast cell immune synapses, and immune interactions in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, The Immune Synapse: Methods and Protocols will be of interest to immunologists and, at a more general level, to cell biologists, biophysicists and computational biologists.

1. The Immune Synapse: Past, Present, and Future

Michael L Dustin and Cosima T Baldari

2. Analyzing Actin Dynamics of the Immunological Synapse

Katarzyna I Jankowska and Janis K Burkhardt

3. Analysis of Microtubules and Microtubule-Organizing Centre at the Immune Synapse

Noelia Blas-Rus, Eugenio Bustos-Mor?n, Francisco S?nchez-Madrid, and Noa B Mart?n-C?freces

4. Analyzing the Dynamics of Signaling Microclusters

Akiko Hashimoto-Tane, Tadashi Yokosuka, and Takashi Saito

5. Reconstitution of TCR Signaling using Supported Lipid Bilayers

Xiaolei Su, Jonathon A Ditlev, Michael K Rosen, and Ronald D Vale

6. Plasma Membrane Sheets for Studies of B Cell Antigen Internalization From Immune Synapses