Immunohistochemistry Basics and Methods [Paperback]

This concise yet comprehensive guide to the methods and protocols of immunohistochemistry covers established techniques and current developments in the field such as the use of epitope tags, multiple immunolabeling and diagnostic immunohistochemistry.

Antibodies for Immunohistochemistry.- Antibody Labeling and the Choice of the Label.- Probes Processing in Immunohistochemistry.- Working with Antibodies.- Background Staining, Autofluorescence and Blocking Steps.- Immunostaining Enhancement.- Multiple Multicolor Immunoenzyme Staining.- Multiple Immunofluorescence Staining.- Antigen Detection on Tissues Using Primary Antibody Raised in the Same Species.- Probes for Staining Specific Cellular Organelles.- The Use of Epitope Tags in Histochemistry.- Immunohistochemistry at the Ultrastructural Level.- Diagnostic Immunohistochemistry.- A Picture Is Worth a Thousand Words.

From the reviews:

Immunohistochemistry: Basics and Methods, a new book that aims to make the technique accessible to a wide audience. & The text is easy to read and practical & . this book is an effective primer on the science and technique of immunohistochemistry. I would recommend & particularly to those who are themselves doing a significant amount of hands-on immunohistochemistry or for those seeking to develop the technique in their department. Indeed anyone involved in the interpretation of immunohistochemistry would benefit from reading this book. (Colan Ho-Yen, ACP News, Spring, 2011)

Each protocol described has been proved by the authors; many of the methods are routinely used in daily practice in their institute

Well established methods as well as easy-to-adopt protocols reflecting recent developments in immunohistochemistry are provided

All practical steps are clearly described, including lists of all necessary material

 

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