Immunoproteomics Methods and Protocols [Paperback]

Providing current diverse approaches and techniques used to study the immunoproteome, Immunoproteomics: Methods and Protocols collects chapters from key researchers that deliver information to be used in diagnostics, disease progression, and vaccine correlates of protection analysis, to name but a few. This detailed volume includes techniques used for the study of the antibody targets of bacterial pathogens, viruses, and cancer, mass spectrometry-based approaches to characterize T-cell epitopes, chapters on detection and relative quantification of cytokines in serum, as well as in silico prediction of epitopes using sequence-based or modeling approaches.? Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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Practical and thorough, Immunoproteomics: Methods and Protocols aids researchers in transferring these techniques to their own laboratories in addition to providing a reference to guide researchers toward appropriate techniques.

Providing diverse, up-to-date approaches and techniques for studying the immunoproteome, this Methods in Molecular Biology book offers materials lists, step-by-step reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

1. Introduction to the Immune System

??????????? Scott McComb, Aude Thiriot, Lakshmi Krishnan, and Felicity Stark

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2. Immunoproteomics: Current Technology and Applications

??????????? Kelly M. Fulton and Susan M. Twine

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3. Antigen Identification Using SEREX

??????????? Ugur Sahin and lÓ4